specific radioactivity meaning in English
比放射性活度
Examples
- Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions . so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor . in this study , radiolabelled ligand is used to investigate the binding characteristic of cam and a . thaliana protoplasts . and chemical crosslinking is employed to explore binding proteins in the membrane . at first , ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e . coli . . sds - page and autoradiograph indicated high - purified , high - specific radioactivity ( 35 ) ~ s - cam was obtained . electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ ( 35 ) s标记的氨基酸混合物喂养工程菌成功地制备了~ ( 35 ) s标记的拟南芥钙调素( ~ ( 35 ) s - cam ) ， ~ ( 35 ) s - cam纯度高、放射活度高、 ca ~ ( 2 + )与egta存在时的电泳行为与未标记cam相同，可作为一种高灵敏性的探针用于进行受体学分析实验；用拟南芥种子诱导愈伤，通过酶解制备了大量原生质体。